sodium cacodylate


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sodium cacodylate

[‚sōd·ē·əm ka′käd·əl‚āt]
(organic chemistry)
C2H6 AsNaO2 A herbicide used as a harvest aid. Also known as bollseye (trade name).
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The pellets were postfixed in 2% osmium tetroxide, 0.8% potassium ferricyanide, and 5 mM calcium chloride in 0.1 M sodium cacodylate buffer for 1 hour and washed in sodium cacodylate buffer.
For scanning electron microscopy, whole animals were prefixed overnight in 2% glutaraldehyde and 2% paraformaldehyde, buffered with 0.1 mol [1.sup.-1] sodium cacodylate buffer adjusted to pH 7.4.
Hypobranchial glands were cut and fixed in a Karnovsky fixative (4% glutaraldehyde 2% paraformaldehyde in 0.1 M sodium cacodylate buffer), pH 7.8 at 4[degrees]C, for overnight, and washed in 0.1 M sodium cacodylate buffer.
After fixation midgut fragments were post-fixed in 1% osmium tetroxide and 0.8% potassium ferricyanide in 0.1 M sodium cacodylate pH 7.2 for 1 hour, dehydrated in graded acetone series and embedded in Epon 812.
The tissue was then rinsed, trimmed into [1-mm.sup.2] pieces with a razor blade, and stored in 0.1 M sodium cacodylate buffer.
It was first postfixed in 1.0% osmium tetraoxide in 0.1 M sodium cacodylate buffer, followed by dehydration in graded ethanols, and then critical point dried using a Sorvall critical point dryer (Newtown, CT).
The whole tongues were fixed in modified Karnovsky solution, containing 2.5 % glutaraldehyde and 2 % paraformaldehyde in 0.1 M sodium cacodylate buffer (pH 7.4), for three days, at 4 [degrees]C; small samples (approximately 2 [mm.sup.2]) of tissue, dissected from some areas (from the tip to the base) of the dorsal tongues were obtained.
They were washed in 0.1 M sodium cacodylate buffer (pH 7.4) and treated with 8 N hydrochloric acid, at 60 [degrees]C, for 1 h, to remove any extracellular mucous substance from the lingual surface (Estecondo et al., 2001).
The cells were fixed by adding a drop of 2.5% glutaraldehyde in 0.05 M sodium cacodylate buffer, pH 7.4, osmolality 357 mmol/kg, and allowed to settle on the Cell-Tak-coated coverslips in a moisture chamber for 24 to 36 h at RT.
In the rinses with sodium cacodylate buffer and 25% and 50% ethanol, a few bipolar and multipolar neurons with long processes (some more than 300 [[micro]meter] in length) were observed, measured, and photographed.
According to the method of Einsenman and Alfert (1982), gametes were prefixed for 10 min in seawater containing 1% glutaraldehyde (Nacalai Tesque Inc.) and 0.05% osmium tetroxide (TAAB) or in 0.2 M sodium cacodylate buffer (pH 7.2) containing 1% glutaraldehyde and 0.05% osmium tetroxide, 0.
For cytochemistry, the hemolymph was drawn into a syringe containing 0.5 ml of 1% glutar-aldebyde, 1% saccharose in sodium cacodylate buffer (0.2 M, pH 7.0).