[alpha]-amylase was assayed by adding 1 ml of enzyme to 1 ml soluble starch
(1%) in an acetate buffer pH 5, and incubated at 50[degrees]C for 15 min.
Commonwealth Scientific & Industrial Research Organisation (Campbell, Australia), The Austrailian National University (Acton, Australia) and Biogemma SAS (Paris, France) have patente a nucleic acid sequence encoding an enzyme of the starch biosynthetic pathway in a cereal plant, wherein the enzyme is selected from the group consisting of starch branching enzyme I, starch branching enzyme II, starch soluble synthase I, and debranching enzyme, with the provisio that the enzyme is not soluble starch
synthase I of rice, or starch branching enzyme I of rice or maize.
In wheat grains, losses in starch accumulation caused by heat stress are believed to be linked to a reduction in the activity of soluble starch
synthase (SSS) (Hawker and Jenner, 1993; Jenner et al., 1993; Keeling et al., 1993).
measuring the amount of reducing sugar released during enzymatic hydrolysis of 1% pullulan or soluble starch
in 125 L 50mM acetate buffer (pH
Holt, 1993 Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch
Appropriate amount of soluble starch
and glucose was added to the mixture and homogenized thoroughly for 20 minutes.
Meanwhile, high temperature at filling stage could also increase the amount of long B chains of amylopectin and decrease short B chains (Asaoka et al., 1985), which is more dominantly attributable to the changing activities of soluble starch
synthase (SSS), starch branching enzyme (SBE) and starch debranching enzyme (DBE) in filling endosperms, rather than the limitation in sucrose supply from photosynthesis tissues and its cleavage metabolism in non-photosynthesis tissues.
The different concentrations of soluble starch
(1.0,2.0,2.5) were added to othere three flasks contained B2medium.
Gels were incubated overnight in 100 mM citrate-NaOH buffer (pH 6.0) containing 20 mM glucose-1-phosphate and 0.5 mg [mL.sup.-1] soluble starch
(BDH Chemicals, Poole, England).
86 being periodically cultivated in the optimized medium containing 4% soluble starch
and 0.5% CaCl2 showed an 8 fold increase in amylolytic activity as compared to the enzyme activity in primary medium.
The substrate specificity of the enzyme from both the crude extract and post dialysis solution (ammonium salt precipitation) upon 3 different substrates: soluble starch
, glycogen and dextrin.