With two ROS1-positive patients remaining on treatment for more than 2 years, we believe the results we announced today further distinguish repotrectinib, and are supportive of our ongoing, multi-cohort registrational Phase 2 TRIDENT-1 Study."With TPX-0046, we have demonstrated strong preclinical potency in Ba/F3 cells with the mutated KIF5B RET fusion gene and against multiple solvent front
mutations that may arise from other investigational RET inhibitors and lead to treatment resistance.
Repotrectinib is a rationally designed, low molecular weight, macrocyclic TKI that is much smaller than current ROS1, TRK family and ALK inhibitors with the objective to systematically overcome the clinically acquired resistance mutations of ROS1, TRK family and ALK kinases, especially the gatekeeper and solvent front
An Olympus-BHT optical microscope (Shinjuku-ku, Tokyo, Japan) was used to measure the penetration distance of the sharp, solvent front
. The cleavage surface was also observed by an optical microscope.
The plate was removed after the solvent reached the solvent front
. The chromatogram was then sprayed with the spraying reagent (mixture of 1% Iron (III) Chloride and 1% Potassium Ferricyanide).
The solvent front
was marked and [R.sub.f] value was calculated.
In the 1980s, I remember a sales rep coming to me and asking how he could break it to someone that the work that they had done using LC-UV was negated by the fact that they were measuring the solvent front
rather than the compound of interest.
When micellar solutions of anionic surfactants (SDS) were applied the protein fraction transferred together with the solvent front
thus separation of caffeine spot from the serum was observed.
After the solvent front
has traveled up the plate to at least 5 cm, remove and dry the plate in the hood to remove all the vapors.
In PC, 99mTc labeled isolink kit remain at the bottom while and free 99mTc moves along with solvent front
. In ITLC, 99mTc(CO)3-ligand migrate with solvent front
and free pertechnetate remained at the origin.
The plates was kept in the TLC tank till the solvent front
reached the top of TLC plate.
On the solvent front
a lot of misinformation has been created about toxicological risks and/or flammability factors.
Substances close to the solvent front
exhibited the typical quinidine-like effect on frog hearts with decreases in both rate and force of contraction.