sonicate

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sonicate

[′sän·ə‚kāt]
(engineering)
To apply high-frequency sound waves to matter.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
In order to achieve these structures, some contribution energy, for example, mechanical (stirring or sonicates) or heat, are essential.
The same result was observed for broth used for phage propagation and appropriate bacterial sonicates (Figures 2(a), 2(c)-2(f)) except sonicate of P.
Purification of fusion GST-SK Total recovery Steps Protein SK Specific Fold % (mg) (IU) activity purified Yield Initial 12.7 120500 9488 -- -- (Sonicate) Elution 4.25 113270 26652 2.8 94 (Purified) Discussion
Serological testes used for the detection of CT crosss-reactive material in the culture filtrates were (i) ganglioside GM1 ELISA in which ELISA plate coated with GM1 ganglioside was reacted with culture supernatant and rabbit polyclonal CT antibody, (ii) direct ELISA in which ELISA plate was directly coated with bacterial sonicate and reacted with CT antibody, and (iii) immunoblot assay in which bacteria-free culture filtrate, crude outer membrane protein (OMP), or sarcosyl-purified OMP was separated by SDS-PAGE, transferred to nitrocellulose membrane and probed with rabbit anti-CT antibody.
Effect of pinealectomy and pineal incubation medium and sonicates on insulin release by isolated pancreatic islets in vitro.
DNA was isolated from the nuclear pellet of liver sonicates (~5 mg of starting tissue) by adding 600 [micro]L of a solution containing 25 mmol/L EDTA and 20 g/L sodium dodecyl sulfate; 10 [micro]L proteinase K (stock solution 10 g/L) was added before incubating for 2 h at 60 [degrees]C.
Twenty-eight critically ill patients admitted to the intensive care unit were randomly assigned to receive 1 of 3 treatments daily for 7 days: 1) placebo, 2) viable probiotics, or 3) equivalent probiotic sonicates. MODS scores and systemic concentrations of immunoglobulin (Ig) A and IgG were measured on days -1, 4, and 7, and intestinal permeability measurements were taken daily.
The clay mimic crystals were sonicated in a solution of the individual organic molecules, most of which contain polar groups, and tests were taken of the sonicates to determine if intercalation occurred.
The sonicates were then centrifuged at 13,000g for 5 min, and the supernatant was transferred to black 96-well microplates (Packard Instrument Co., Meriden, CT).
We used purified EspA, which was confirmed by SDS-PAGE to be a single band with no detectable contaminants, while Parissi-Crivelli used sonicates of organisms expressing an unknown amount of EspA on a plasmid and subtracted as background the sonicates of the vector bacteria lacking the gene for EspA.