Determination of magnesium in blood serum was carried out on the device spectrofluorimeter
type AF-610 (ARAYLEIGHL TD Shanghais ANTAI Diagnostics Co, LTD) with sets of test systems BioLab (Russia).
Permeabilization of the liposomal membrane was assayed by incubating the liposome with VCC for varying lengths of time and quantifying the released calceine in a spectrofluorimeter
(excitation wavelength: 485 nm; emission wavelength: 520 nm).
Membrane fluidity was determined using an FP-550 polarization spectrofluorimeter
(Jasco, Japan) as previously described (Lindi et al.
Moreover, the spectra of the derivatives of Cys with SBD-F and SBD-BF, recorded with a Perkin-Elmer MPF 44 A spectrofluorimeter
, were practically superimposable, suggesting that our new reagent SBD-BF is a suitable precolumn derivatization reagent for reversed-phase HPLC fluorometric determination of thiols.
Luminescence spectra and luminescence excitation spectra were measured at room temperature with a HITACHI 650-60 spectrofluorimeter
connected through a data acquisition system to a computer.
Mitochondrial respiration was carried out in the fiberoptic spectrofluorimeter
in the presence of different substrates: succinate, 4.
Skin hypericin content is determined through fluorescence emission with a fiber optic attachment to a spectrofluorimeter
The emission spectra were measured using a custom-made calibrated spectrofluorimeter
described previously (6).
A fibre-optic spectrofluorimeter
with an optical layout based on an epifluorescence microscope provides an excellent combination of sensitivity and ease of use.
The fluorescent metabolite 7-hydroxycoumarin was measured using a Perkin--Elmer spectrofluorimeter
, excitation wavelength 370nm; emission wavelength 474 nm.
Tenders invited for Ss spectrofluorimeter
The excitation wavelength was set at 360nm and the emission wavelength to 450nm using a spectrofluorimeter
(Shimadzu RF-5301PC, Japan).