Spectrophotometry


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spectrophotometry

[¦spek·trō·fə′täm·ə·trē]
(analytical chemistry)
A method of chemical analysis based on the absorption or attenuation by matter of electromagnetic radiation of a specified wavelength or frequency. The radiation interacts with specific features of the molecular species being determined, such as the vibrational or rotational motions of the chemical bonds. The radiation can also interact with specific atoms or the whole molecule, for example, by causing the molecule to change its electronic energy state.

Spectrophotometry

 

a branch of measurement technology that draws on spectrometry, photometry, and metrology in developing methods and instruments for the quantitative measurement of spectral absorptance, reflectance, emissivity, and luminance as quantities characterizing media, coatings, surfaces, and radiators.

References in periodicals archive ?
As seen from Table 5, the residue content of styrene monomers analyzed from polystyrene latex samples was 0.217 for analysis with UV/visible spectrophotometry. This small amount was compared with the results of the GC method because of the possibility of the impurity which absence in GC gas or the presence of dimers and trimers that were difficult to avoid disturbing the absorption.
The development of the analytical methods by spectrophotometry in the mid-infrared region has been successfully used in the quantification of other drugs [18, 21-36].
Spectrophotometry to measure cffDNA is a cheap method, but it may not be intensive enough or we need larger patient groups for searching.
This article is structured, first, by entering a brief identified issue and contextualization of the standards which must adhere to the developed method; it shows then, the research base for the development; then, its validation materials and methods from spectrophotometry and metrology; then the analysis and design of software which automate the method by own statistical analysis of metrology and statistics are shown.
Second Derivative Spectrophotometry. Figures 8 and 9 show the second derivative absorption spectra of DA and [DELTA][lambda] at pH 9.2 after 5 min with a range of concentrations 2-50 [micro]M and 2-2.4 [micro]M, respectively.
Calculated F values were 0.77 and 2.14 for CZE and UV spectrophotometry, respectively.
In this present work, selective, sensitive, simple, and low-cost procedures were developed for the simultaneous determination of Carmoisine (C) and Ponceau 4R (P) in synthetic mixture and drink samples by using derivative spectrophotometry (DSM), ratio derivative spectrophotometry (RDM), derivative differential spectrophotometry (DDM), and compensation method (CM).
This paper describes the development and subsequent validation of a novel, simple, and rapid spectrophotometric method "Sequential Spectrophotometry" for simultaneous quantitation of ternary mixtures.
A later paper described using methylene blue as the working chemical for clay detection, but proposed a new method based on spectrophotometry instead of the standard drop test.
Concentration of 3-hydroxybutyrate is usually determined through methods such as chromatography, isotopic measurements, and spectrophotometry. These methods, however, are usually time-consuming and require a number of processing stages.
Five of these 6 neonates had TSB levels more than 20 mg/dl by direct spectrophotometry (capillary sample) and diazo method (clot sample), and one of them had a TSB level of 18.9 mg/dl.
The most commonly used methods to monitor dyes during the dyeing process are spectrophotometry and voltammetry (BERGAMINI et al., 2005; CARBO et al., 2010; CATANHO et al., 2006; THOMAS; AURORA, 2006; ZANONI et al., 2006).