Spectrophotometry


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spectrophotometry

[¦spek·trō·fə′täm·ə·trē]
(analytical chemistry)
A method of chemical analysis based on the absorption or attenuation by matter of electromagnetic radiation of a specified wavelength or frequency. The radiation interacts with specific features of the molecular species being determined, such as the vibrational or rotational motions of the chemical bonds. The radiation can also interact with specific atoms or the whole molecule, for example, by causing the molecule to change its electronic energy state.

Spectrophotometry

 

a branch of measurement technology that draws on spectrometry, photometry, and metrology in developing methods and instruments for the quantitative measurement of spectral absorptance, reflectance, emissivity, and luminance as quantities characterizing media, coatings, surfaces, and radiators.

References in periodicals archive ?
For ultraviolet-visible spectrophotometry, their linearity ranges were observed to be 0.
Due to the nature of spectrophotometry, a given liquid will have different levels of absorbency at different wavelengths.
Third, serum bilirubin was measured using direct spectrophotometry rather than the ideal method of high performance liquid chromatography (HPLC).
Simultaneous determination of lamivudine and stavudine in antiretroviral fixed dose combinations by first derivative spectrophotometry and high performance liquid chromatography.
At the optimum experimental conditions of the reaction of the reagent HOTT with mercury (II) in the aqueous solution of pH 4-6, the effective molar absorptivity ([epsilon]) calculated from Beer's-Lambert plot and the Sandell's sensitivity index [22] of the mercury (II)-complex with and without the use of [beta]-correction spectrophotometry were found to be 4.
In the current study, we compared COHb results obtained with automated CO-oximetry (Diametrics Medical AVOX 4000) and manual UV spectrophotometry (Hewlett Packard 8453 UV spectrophotometer) (8).
The carotenoid content was estimated using spectrophotometry.
The G7 proof-to-print process is based on the principles of digital imaging, spectrophotometry and computer-to-plate technologies and is a simple calibration process that allows converters to achieve a visual match between their proofing and printing methods.
Metals are analyzed using direct absorption atomic absorption spectrophotometry.
Jenway provides spectrophotometry and electrochemistry equipment, Techne offers temperature control and calibration equipment, and Stuart offers temperature control and motion equipment.