Schroeder et al., "Cardioprotection and lifespan extension by the natural polyamine spermidine
," Nature Medicine, vol.
The eleven biogenic amines studied were ethylamine (ETA), methylamine (MEA), histamine (HIS), serotonin (SER), spermine (SPM), spermidine
(SPD), agmatine (AGM), putrescine (PUT), [beta]-phenylethylamine ([beta]-PEA), cadaverine (CAD), and tyramine (TYM) all of which were supplied by Supelco, Bellefonte, PA, USA, as well as the derivatizing agent dansyl chloride and the internal standard 1,7-diaminoheptane (IS).
Cairo, "Soft TCPTP agonism-novel target to rescue airway epithelial integrity by exogenous spermidine
," Frontiers in Pharmacology, vol.
The plant growth regulator consisting Naphthylacetic acid (NAA) in combination with Kinetin (KN), Spermidine
(Spd) and Benzylaminopurine (BA) were maintained in a growth room at 25 [+ or -] 1[degrees]C.
Their team found that resveratrol and spermidine
increase autophagy by different mechanisms, and that one tenth of the optimal dose of resveratrol or spermidine
can be highly effective when the two substances are given in combination rather than separately.(16) Dr.
The in vivo biotinylated T7 RNAPs were immersed in a reaction mixture containing 2ng/[micro]L 1,000 bp DNA template with T7 promoter and terminator, transcription buffer (40 mM Tris-HCl pH 7.9, 6mM Mg[Cl.sub.2], and 10 mM NaCl), 2mM spermidine
, 10 mM DTT, 2mM of each rNTP, 2units/[micro]L RNasin, and 2.6 [micro]M Bio-T7 RNAP.
(1996) reported the accumulation of [N.sup.4]-Hexanoyl spermidine
, a polyamine-related compound during the ovary and petal senescence in pea.
Samples of muscular tissue removed from each individual were macerated in liquid nitrogen and homogenized in PS buffer (Tris-HCl 0.2 M, EDTA 30 mM, SDS 2% e Sucrose 5%) TH buffer (Tris-HCl 10 mM, NaCl 60 mM, EDTA 10 mM, Sucrose 5%, Spermine 0.15 mM and Spermidine
0.15 mM) pH 8.0 e protease K (20 [micro]g [micro][L.sup.-1]) for 90 minutes in bain-marie at 37[degrees]C.
The organic compound spermidine
is a polyamine and is found in all living cells.
A new study says that spermidine
reversed the effects of growing old during lab tests on mice and worms.
The exact mechanism of chloroquine-induced psychosis is not known but the role of different neurotransmitter systems--polyamines (especially spermidine
) excess, dopamine excess, acetylcholine imbalance and prostaglandin-E antagonism have been postulated.
Polyamines such as putrescine, spermidine
, spermine, cadaverine and agmatine are plant growth regulators involved in all growth and development process of plants (Kumar et al., 1997).