spheroplast


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spheroplast

[′sfir·ə‚plast]
(cell and molecular biology)
A plant cell which possesses only a partial or modified cell wall.
(microbiology)
A bacterial cell that assumes a spherical shape due to partial or complete absence of the wall.
References in periodicals archive ?
One of the labs working with spheroplasts was led by Ching Kung, PhD, at the University of Wisconsin-Madison.
The observation and comparison of W303 cells and spheroplast W303 were successfully performed using ESEM.
This was probably because multiple nuclei were present in each spheroplast, and therefore a single pyrG[DELTA]0 locus could not prevent production of the suicide inhibitor FdUMP.
de Cock, "Lipopolysaccharide transport to the bacterial outer membrane in spheroplasts," Journal of Biological Chemistry, vol.
To examine the effect of these mutations on the gating threshold of MscS, the channel activities were measured while applying negative pressure through a patch pipette to the inside-out membrane patch of the giant spheroplasts expressing the wild-type or mutant MscS.
The resulting spheroplasts were harvested by centrifugation (13000xg at 4[degrees]C) and the supernatant, referred to as the periplasmic fraction, was recovered.
Samples were incubated for 20 min at 30[degrees]C with gentle agitation to facilitate the formation of spheroplasts. Total RNA was purified on a robotic QIAcube workstation (Qiagen) using the RNeasy Mini Kit (Qiagen), following the manufacturer's instructions, including DNase treatment.
Susceptibility of bacteria and complete spheroplasts of Escherichia coli, Pseudomonas aeruginosa and Salmonella Typhi to rolliniastatin-2.
The spheroplasts were washed once with sorbitol solution and resuspended in 50mM sodium phosphate buffer, pH 8.0, containing 300mM NaCl and 2mM [beta]-mercaptoethanol.
Even when the spirochete lacks or has too few components that condition its growth, it is able to produce endospores, such as cysts (spheroplasts, L forms) or the so called blebs (gemmae) [2, 3].
(14.) Davies, A, Bentley, M, Field, BS, "Comparison of the Action of Vantocil, Cetrimide, and Chlorhexidine on Escherichia coli and Its Spheroplasts and the Protoplasts of Gram-Positive Bacteria." J.
Briefly, fungal suspensions were centrifuged for 10 min at 3000g, and 1 mL of lyticase buffer was added to generate spheroplasts [10 kU/L recombinant lyticase (Sigma), 50 mmol/L Tris,1 mmol/L EDTA, 2 mL/L ([beta]-mercaptoethanol].