spore stain

spore stain

[′spȯr ‚stān]
(microbiology)
A type of staining technique that utilizes the fact that the spore does not take up dyes readily but, once stained, it resists decolorization. The differentiating agent used may be a dilute solution of an organic acid, an acid dye, or another basic dye.
References in periodicals archive ?
Objects with possible contamination were evaluated for spores with the spore stain (Malachite green) by wet-mount-phase microscopy and were cultured for Bacillus anthracis under modified biosafety level-3 conditions (2).
A The spores of Bacillus anthracis do not fluoresce under ultraviolet light, but can be visualized by phase contrast microscopy or after staining with a spore stain. Most routine clinical laboratories (Level A) should not attempt to examine environmental samples (unknown powders suspected of containing anthrax spores) because the potential for aerosolization of the material is so great.
A spore stain may be used, but often the suspected powder does not adhere to the slide.