Evaluation of the FASTPlaque TB assay for direct detection of Mycobacterium tuberculosis in sputum specimens
. Int J Tuberc Lung Dis 2002; 6: 635-640.
Four of these cases were unable to produce sputum specimens
The patient can be presumed to have NTM if a second sputum specimen
is smear-positive, NAA-negative, and has no inhibitors detected.
From July 1990 through August 1991, the inmate was transferred between three different state prisons, and sputum specimens
obtained during that time were predominately smear- and/or culture-positive.
In both instances, chest radiographs showed no new changes, sputum specimens
were negative for acid-fast bacilli (AFB) by microscopy and culture, and she was treated for presumptive Pneumocystis pneumonia and showed clinical improvement.
from each participant was identified with a unique numbering system followed by A or B indicating soon after the patient wakes up and before any antiseptic mouth-wash use, respectively.
On HD 8 a sputum specimen
was sent for routine bacterial, fungal, and acid-fast cultures.
Direct smears were prepared from each sputum specimen
for microscopic investigation using AFB stain by the ZiehlNeelsen (ZN) technique (ST Reagensia Company, Jakarta, Indonesia) and interpreted according to standard guidelines.
Smear positive pulmonary tuberculosis defined as patient with at least two sputum specimens
positive for acid-fast bacilli (AFB) by microscopy, or radiographic abnormalities consistent with pulmonary tuberculosis with one sputum specimen
positive for AFB11.
However, the included criteria of sputum specimen
for microscopic examination were not described in this study.[sup]
The first aliquot of the sputum specimen
[2-3 mL in 14 mL BD Falcon [TM] centrifuge tube (Becton Dickinson, Franklin Lakes, NJ, USA)] was subjected to direct AFB smear examination and N-acetyl L cystiene (NALC)-sodium hydroxide (NaOH) decontamination method (final NaOH concentration 1%).
volumes received from children for Xpert testing are shown in Table 1.