With liquid chromatographic enantiomer separations the chiral selector may either be added to the (aqueous) mobile phase or may be contained in the stationary phase
, preferably fixed to the surface either by SiO bonds or as substituents in polymers, which form an immobilized coating on such a surface.
A gas chromatograph consists of an injection port, a flowing mobile phase, a separation column containing the stationary phase
and a detector.
Once growth enters a stationary phase
in the fermentation cycle, the oxygen injection can be reduced in line with the requirement.
The mixture is moved physically past a stationary phase
, which may be either a solid or liquid immobilized in the pores of a solid.
In the first report of global gene expression monitoring in a bacterial pathogen, oligonucleotide microarrays were used to measure the relative transcript levels of 100 Streptococcus pneumoniae genes during the development of natural competence and during stationary phase
In particular, it is possible to have retention of a macromolecule within a chromatographic column as long as a single structure unit may positively interact with the stationary phase
, whereas migration requires all structural units being in the mobile phase.
During this so-called stationary phase
, some bacteria shield their DNA from degradation by embedding the genetic material within crystals of a protein called Dps, Abraham Minsky of the Weizmann Institute of Science in Rehovot, Israel, and his colleagues report in the July 1 NATURE.
The term "immunoaffinity chromatography" (IAC) is used for an affinity chromatographic method in which the stationary phase
consists of an antibody or antibody-related reagent (5, 35).
Then, through shortage of nutrients and accumulation of inhibitory metabolic products, the stationary phase
(stagnation of cell growth) is reached.
Rasheed, ICP Spectrometric-Vis Separation of Cerium (IV)- Desferal Complex Using 4-Vinylbenzyl-Dimethylammonio Pentanesulfonate Zwitterionic Stationary Phase
That is, a normal stationary phase
(silica gel) developed with a nonaqueous mobile phase, which separates preferably nonpolar components and, in parallel, a reverse stationary phase
(octadecyl silica) developed with an aqueous phase, used to separate preferably polar components [24, 25].
But in batch experiments, with substrate consumption and cells step into stationary phase
, Cells appear the phenomenon of accelerated decline, resulting in their own energy and biomass fell rapidly, the reason is by H2O2 and the accumulation of intermediate product Because the continuous culture conditions cells logarithmic phase can effectively extend, and maintain a relatively stable cell activity and growth rate.