However in our laboratory setup, histochemical staining for enzymes such as alkaline phosphatase, phosphorylase, succinic dehydrogenase
, indoxyl esterase, and acid phosphatase was not available; they were not performed.
We investigated the mechanism of the action of the Huangjingzanyu optimized formula on mitochondrial energy metabolism by measuring sperm adenosine triphosphate (ATP) content and changes in succinic dehydrogenase (SDH) and lactate dehydrogenase-C4 (LDH-C4) activities, Compared to the model group, the sperm ATP content was significantly increased in the high- and moderate dose Huangjingzanyu optimized formula.
Succinic dehydrogenase (SDH) and lactate dehydrogenase-C4 (LDH-C4) are closely related to sperm production, metabolism, and energy acquisition (Li and Li, 2011; Guo et al.
Effect of omethoate on the testis succinic dehydrogenase (SDH) and the quality of sperm in male mice.
Furthermore, succinic dehydrogenase and alkaline phosphatase activity were also decreased.
Standard dehydrogenase technique with tetrazolium solution was applied to demonstrate activity of succinic dehydrogenase which appeared as bluish- purple formazan deposits, Gomori's calcium method was applied to demonstrate activity of alkaline phosphatase which appeared in the form of brownish-black colour, and Gomori's lead method was applied for acid phosphatase which took the brownish-black colour (Bancroft & Gamble).
Strong succinic dehydrogenase activity was detected in the cytoplasm of the hepatocytes, while no activity was detected in the regions of the nuclei (Fig.
The activity of succinic dehydrogenase in the hepatocytes was progressively decreased and became very weak in some areas (Fig 2D).