They are using the state-of-the-art antibiotic of that time, sulfanilamide
, pouring it on the fake wound.
NO generated in the culture medium was measured with the Griess reagent (0.1% N-(1-naphthyl) ethylenediamide dihydrochloride and 1% sulfanilamide
in 5% phosphoric acid); NO production was determined directly from this measurement.
He picks up the story with the post-war achievements of German chemists who finally discovered that the combination of an azo dye and a sulfanilamide
side chain was able to kill the family of Streptococci bacteria.
Taste of raspberries, taste of death--The 1937 elixir sulfanilamide
50 [micro]L of culture medium was transferred to one well of ELISA plate in triplicate followed by addition of 50 [micro]L of sulfanilamide
solution ([C.sub.6][H.sub.8][N.sub.2][O.sub.2]S, 1% sulfanilamide
in 5% phosphoric acid).
Ganesh, "Electrochemical detection of sulfanilamide
using pencil graphite electrode based on molecular imprinting technology," Electroanalysis, vol.
Briefly, N-(1-naphthyl)-ethylenediamine (NED) (Sigma[R], USA) was prepared at 0.1% and sulfanilamide
(Sigma[R], USA) at 1%, both with phosphoric acid at 2.5% as a diluent.
the concentration of nitrite (N[O.sub.2]), a soluble oxidation product of NO, in the culture media was measured using Gries reagent (0.1%N-l-napthylethylenediamine dihydrochloride and 1% sulfanilamide
in 5% phosphoric acid) (Ha et al., 2010).
and N-1-naphtylethylenediamine dihydrochloride solutions were added to the samples, respectively.
The biopsied area was closed with surgical sutures, and cleaned with betadine topical solution and Negasunt spray (Coumaphos 30 g, Propoxur 20 g, and Sulfanilamide
50 g/kg; Bayer Korea, Korea) and Pink spray (10 mg mercuric oxide yellow and 1 mg prednisolone/kg; Komipharm International, Siheung, Korea).
(13) Later, in response to the death of over one hundred people, mostly children, after taking elixir sulfanilamide
, the Food, Drug, and Cosmetic Act of 1938 became law.