supernatant


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supernatant

[‚sü·pər′nāt·ənt]
(biochemistry)
The overlying soluble liquid fraction of a sample that remains after precipitation of the insoluble solid component by centrifugation.
References in periodicals archive ?
The levels of FSH, LH and cAMP in the cell supernatant in Cetrorelix(-) group were significantly decreased compared to the blank(-) group (p<0.05), while the LH level in the CSF(-) group was significantly increased (p<0.05).
But, the lysis of RBCs was observed in moderate intensity by hemagglutination test for non-heated bacterial supernatant in twofold dilution 1/10-1/320, while, agglutination appeared after treating the sample with heated supernatant which indicates that RBCs did not lyse (Table 2).
In the EBL supernatant, the 20-CuU and 10-CuU treatments achieved significantly greater N[O.sub.3.sup.-]-N levels compared with urea-only by day 2.
The abovementioned cultured bacteria solution was centrifuged at 10000 rpm for 10 minutes, and the supernatant fluid was discarded.
Spraying method: Supernatant of fungal fermentation broth and partially purified mixture protein in the volume of 50 ul/cm2, were sprayed on Sinapis arvensis and Triticum aestivum with 3 replications, in sterile conditions and with minimal physical stress.
Biological effects of the QELBY powder and their powder supernatants were examined via the "contact" and "noncontact" model experiments.
The PDGF-BB and TGF-[[beta].sub.1] concentrations in PRG supernatants, PRP lysates, and plasma were determined in duplicate by sandwich ELISA developed with commercial antibodies for human TGF-[[beta].sub.1] (Human TGF-[[beta].sub.1], DY240E, R&D Systems, Inc., MN, USA) and PDGF-BB (Human PDGF-BB, DY220, R&D Systems, Inc., MN, USA), because these mammalian GFs are highly homologous between both species (90% identity or more) and similar ELISA antibodies have been used to measure these polypeptides in cow samples [24-27].
The release of IL- 1[beta] to the cell culture supernatant was measured by ELISA.
The protein content of the supernatant fraction was adjusted to 3.57 mg/0.8 mls and applied to a Sepharose CL-6B filtration column (30 cm x 1.5 cm i.d.) preequilibrated with TBS.
Plasma RNA, exosome RNA, and supernatant RNA were respectively pooled from 10 healthy samples for small RNA library preparation and Illumina Hiseq2500 sequencing as described in our previous study [23].