was used to analyze for interacting proteins by western blot analysis.
AM-conditioned media were collected and centrifuged, and the supernates
were saved in aliquots at -80[degrees]C for further analysis of cytokines.
For virus isolation, frozen tissues were homogenized, the supernate
filtered through a 0.22-[micro]m filter, and plated on green sea turtle embryo fibroblasts (Moore et al., 1997).
Though a harsh extraction procedure, previous bioassays indicated (i) that the biologically active compounds remained in tissue residual when extracted with cold methanol, (ii) that some of the biologically active compounds remained in the tissue residual and some were captured in the supernate
when extracted with room temperature methanol for 24 h, and (iii) that the biologically active compounds were pulled from the tissue residual and captured in the supernate
when exposed to boiling methanol by the modified Coseteng and Lee method presented above (Warnock, 1998).
The bursa homogenate was centrifuged at 1,500 xg for 20 minutes, and the supernate
was collected and stored frozen at -70[grados]c until it was used as inoculum.
Sodium (Na) Greater than 30 mEq/L Specific gravity Fixed near 1.010 Osmolarity Fixed near 287 mOsm Ammonium sulfate precipitate test Supernate
will be red-brown; reagent test strip will be positive for blood.
A recently developed immunologic assay isolates a subset of TRL that are enriched in apo E and display classic remnant-like characteristics.[75-77] These unbound remnants remain in the supernate
and have been identified as remnant-like particles (RLP).
The slurry was transferred to a centrifuge tube and the undigested residue separated from the supernate
. The supernate
was taken to near dryness and then diluted with deionized water.
The uranium depleted supernate
solution overflows the thickener to surge tanks for disposal via a deep injection well.
Although none of the sediments were analyzed for herbicide content, it is not unreasonable to assume that the higher concentrations of atrazine and alachlor in the samples from the plowed treatment was partially due to equilibration of the supernate
with the sediment from the soil surface that had been directly exposed to the spray application.
The remaining polymers were cleaned by dissolution of the polymer in THF, centrifugation of the solutions at 4000 rpm ([similar to] 2000 g force), and precipitation of the supernate
solution into methanol, a non-solvent.
was discarded and the sediment was resuspended to 1.0 mL with phosphate-buffered saline.