supravital


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supravital

[¦sü·prə′vīd·əl]
(biology)
Pertaining to the staining of living cells after removal from a living animal or of still living cells from a recently killed animal.
References in periodicals archive ?
Tanto la vitalidad evaluada por la tecnica supravital de eosina/nigrosina (Figura 2) como la vitalidad evaluada por azul tripan-DIC, fue significativamente mayor en las muestras congeladas con el metodo propuesto que en grupo control (Figura 3).
Other red cell inclusions such as Heinz bodies and Haemoglobin H inclusions can only be appreciated with supravital staining (reticulocyte preparations).
Key Words: Fine needle aspiration cytology, Supravital stain, Breast, Thyroid, Lymph nodes, salivary glands.
The percentage of normal nonapoptotic cells and apoptotic cells was measured by double supravital staining with Annexin-V and propidium iodide (PI) following manufacturer's instruction, using an annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (KeyGen, Nanjing, China).
Spermatozoa viability (supravital staining technique) and PMI rates (HOS solution: 75 mOsm/kg) were evaluated as per method of Khan and Ijaz, (2008) while DNA damage (acridine orange staining technique) and NAR were evaluated as per Tejada et al.
Esta situacion permite postular que soluciones de osmolaridad muy baja, causarian ademas de la respuesta fisiologica de equilibrio osmotico inicial, una mayor cantidad de dano fisico en los espermatozoides en el tiempo; esto considerando que la tincion supravital mide la ruptura de la membrana plasmatica en el espermatozoide muerto--integridad fisica-, mientras que HOST evalua la integridad funcional de la misma--integridad bioquimica(Schrader et al, 1986).
FBC and reticulocyte count (automated Sysmex XE 5000, analyser; Sysmex Incorporated, Japan), blood smears for microscopy (automated Sysmex 1000i slide maker and stainer; Sysmex Incorporated, Japan), and reticulocyte preparation for microscopy (2-hour incubation with 1% Brilliant Cresyl Blue supravital stain; National Health Laboratory Service, South Africa).
Results: Of the 114 aspirates included in our study the diagnostic accuracy by using papanicolaou was 78%, while it was upto 100% with supravital toluidine blue stained smears.
El uso de la prueba HOST-EN en este ensayo se apoya en las investigaciones previas realizadas en los toros [1, 11], donde se observo una correlacion significativa entre la prueba HOST y la viabilidad mediante el uso de la tincion supravital EN.
Manual evaluation of urine sediment may be further enhanced by using supravital stains such as crystal violet/safranin O stain and phase miscroscopy.
The supravital stain trypan-blue was used to distinguish live and dead spermatozoa while giemsa stain was used to evaluate the integrity of the acrosome membrane.