They still didn't present lumen and had interstitial cells, sustentacular
cells, spermatogonias and primary spermatocytes (undergoing cell division).
(a) Positive in the peripherally situated sustentacular
The tumor cells showed a patchy but distinct immunoreactivity to synaptophysin (Figure 2, A) and chromogranin and strong diffuse cytoplasmic membrane immunoreactivity to CD56 (Figure 2, B); immunoreactivity to S100 protein highlighted the sustentacular
These nests of tumor cells were peripherally encircled by spindle cells consistent with sustentacular
The spindle cells in the periphery of tumour nests (Sustentacular
cells) showed strong positivity for S-100 (Figure 8).
D1 and D2 were taken only when D1/D2 [less than or equal to] 0.85, others include epithelial height, testicular weight, sustentacular
cells (Sertoli cells) to germ cell ratio (elongated spermatids) (Qin & Lung, 2002).
Paragangliomas across the various sites share similar histologic and immunohistochemical features, being composed of 2 cell types: chief (type 1) cells and sustentacular
(type 2) cells classically in a Zellballen (organoid or nesting) architectural pattern with a prominent vascular network separating the nests.
Immunohistochemistry is useful in making this distinction (1).The chief and sustentacular
cells of paragangliomas are immunoreactive to neuroendocrine marker stains, such as chromogranin and synaptophysin, but not to epithelial markers, such as cytokeratin (1, 2, 5).
cells between S-100 and cell nests were observed to be positive.
Immunohistochemistry revealed diffuse expression of chromogranin A, synaptophysin and neuron specific enolase, and S100 staining in sustentacular
cells; Ki-67 was <5%.
These cells form a "zellballen" pattern of nests separated by vascular stroma and spindle-shaped sustentacular
cells [28, 43].
The researchers have been working with adult olfactory stem cells that give rise to the neurons that sense odors and other cells, such as sustentacular
cells, that support the neurons.