1-100bp DNA Ladder 2- negative control of RT-PCR(H2O) 3-negative control 4- Sustentacular cells
condition medium after 24 h (50 %) 5- Sustentacular cell
condition medium after 24 h (100 %) 6- Sustentacular cell
condition medium after 48 h(50 %) 7- Sustentacular cell
condition medium after 48 h(100 %) 8-positive control (testicular extraction).
Prolonged dosing resulted in continued spermatocyte degeneration as well as maturation depletion of the spermatid population, leaving tubules containing only sustentacular cells
, spermatogonia and preleptotene spermatocytes.
Immu- nohistochemically, S100 positive sustentacular cells
are not seen as seen in our study in ONB.20
The normal paraganglia consists of nests of chief cells (type1) and sustentacular cells
Phagocytosis of apoptotic bodies by sustentacular cells
within the OE was also evident by ultrastructural examination.
Tyrosine phosphorylation is assumed to play roles in sperm and testosterone productions since phosphorylated proteins have been localized in Interstitial cells (Leydig cells), Sustentacular cells
Sertoli cells) permatogonia, spermatocytes, and spermatids (round and elongated), respectively (Arad-Dann et al., 1993; Chaichun et al., 2017).
The nests are separated by interspersed small blood vessels and sustentacular cells
Paraganglioma is typically positive for synaptophysin and chromogranin, with S100 positivity in the sustentacular cells
The presence of typical Zellballen pattern of arrangement of tumour cells surrounded by sustentacular cells
and fibrovascular stroma favour a diagnosis of pheochromocytoma (Fig.
Flattened sustentacular cells
can sometimes be highlighted in the periphery of the cell nests with S-100 protein.
There was close association with delicate capillaries and S100 positive sustentacular cells
. Appearances were consistent with an extraadrenal paraganglioma.
On immunohistologic analysis, sustentacular cells
stained strongly positive with S-100.