dorsalis, we report the cloning and characterization of the nicotinic acetylcholine receptor alpha6 cDNA and the expression patterns at different developmental stages and in different tagmata of adult flies using quantitative real-time PCR.
The expression levels of Bdor[alpha]6 mRNA relative to expression of [alpha]-tubulin at different developmental stages and in different tagmata of B.
Data from the quantitative real-time PCR for both the developmental expression and different tagmata expression profiles were subjected to LSD test in analysis of variance (ANOVA) (SPSS 12.0 for Windows).
The Bdor[alpha]6 mRNA was found in different tagmata of adult flies.
Several nAChR subunits transcripts from various species were detected in different tagmata. In the present study, Bdor[alpha]6 was found to be present in all tagmatas of adult flies the highest abundance in head followed by thorax and the lowest in abdomen.