Active Ag-NOR occur in telomeric
areas of the short arm in one large and three mediumsized submetacentric pairs of autosomes (Arslan et al.
While the "scabripinnis complex" has been characterized by a preferential centromeric and telomeric
pattern of C-bands (Fernandes and Martins-Santos, 2005; Souza et al.
Additionally long arms of some chromosomes had telomeric
heterochromatin bands (Fig.
In the first step, telomerase added telomeric
repeats (TTAGGG) to the 3' end of biotin labeled synthetic P1-TS and P2, generating PCR products with the telomerase-specific 6 nucleotide increments.
The progressive loss of telomeric
DNA in human somatic (stem) cells is believed to act as a tumour suppressor mechanism that limits clonal proliferation, to prevent clonal dominance and ensure a polyclonal composition of (stem) cells in large, long-lived multicellular organisms.
The gene for achondroplasia maps to the telomeric
region of chromosome 4p.
I proposed that the length of telomeric
DNA, located at the ends of chromosomes consists of repeated sequences, which play a buffer role and should diminish in dividing normal somatic cells at each cell doubling.
Damage within telomeric
regions remains unrepaired and this helps to explain why cells lose their ability to regenerate as they age.
A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric
Telomerase activity using the telomeric
repeat amplification protocol (TRAP) was done using the TRAPeze Telomerase Detection kit S7700 (Chemicon International, Inc.
The mechanism of formation is unclear, but involves the loss of varying extents of the telomeric
ends of the p and q arms, followed by annealing of the "sticky" ends in order to prevent further loss of genetic material.
In this study, the molecular cytogenetic characterization of mitotic chromosomes of the Provence flat oyster or dwarf oyster, Ostrea stentina, was performed through Giemsa staining, chromosome measurements, in situ restriction endonuclease banding, C-banding, fluorescence in situ hybridization with major ribosomal RNA genes (ITS1), and telomeric