DGGE was performed with a 10-30% denaturing gradient adding 210 [micro]L of 10% ammonium persulfate and 10 [micro]L of tetramethylethylenediamine
Tween 80, TRIS Pufferan, Tricine Pufferan, tetramethylethylenediamine
(TEMED), sodium dodecyl sulfate (SDS), mercaptoethanol, bromophenol blue Na-salt, and ammonium persulfate (APS) were supplied from P-Lab (Prague, Czech Republic).
Gel electrophoresis: Native-PAGE was carried by following the method of Laemmli (1970) which included the preparation 4% stacking polyacrylamide gels and 10% resolving polyacrylamide gels followed by polymerisation using the addition of N, N, N', N'- tetramethylethylenediamine
(TEMED) and 10% of ammonium persulfate (APS).
The effect of tetramethylethylenediamine
, (TMEDA), on the yields of products will be presented along with relevant spectral characterization of new 3-substituted aryl 4,5-dihydronaphth[1,2-c]isoxazoles and 1-subsituted aryl 4,5-dihydro-[2,1-c]isoxazoles.
The effects of various organic compounds such as Tetramethylethylenediamine
(TEMED), Marcaptoethanol, Ethylenediaminetetraacetic acid (EDTA) and metal ions including Pb2+, Ca2+, Cl2-, Mn2+, Cu2+ on MnP activity were studied in the concentration of 1-5 mM.