Briefly, 25 L of reaction mixture containing 1 uL of extracted DNA, 12.5 L of PCR Master Mix (Fermentas, Germany), 1 L of forward (10 pmoles/ L) and reverse (10 pmoles/L) primers each (Oligos, GeneLink, USA), and 9.5 L of DEPC water was subjected to thermocycler (ThermoElectron
, Finland) under following conditions; initial denaturation at 94C for 4 min followed by 35 cycles of each of 94C for 30 seconds, annealing at 57C for 1 min and extension at 72C for 1 min.
The samples were analysed by an ICP-AES ICAP 6500 ThermoElectron
for major elements and an ICP-MS XThermoElectronSeries II for trace elements.
The PCR reactions were performed using a ThermoElectron
Px2 thermal cycler.
We used MS/MS with nanoflow liquid chromatography electrospray on a ThermoFinnigan LTQ Orbitrap Hybrid Mass Spectrometer (ThermoElectron
) to look for the targeted peptides for each gel band.
Plasma CK was assayed spectrophotometrically using CK-NAC reagent kits (Thermoelectron
X-ray photoelectron spectroscopy (XPS) experiments of PU hybrid films were performed on a ThermoElectron
ESCALAB 250 spectrometer at room temperature by using an Al and K X-ray source (1486.6 eV).
For LC-MS, full scan electrospray ionization mass spectra of the eluent were obtained using a ThermoElectron
LCQ Advantage 3D Ion trap tandem mass spectrometer (San Jose, CA).
Proteins of individual ticks were reduced with 100 mmol/L dithiothreitol, alkylated with 50 mmol/L iodoacetamide, digested with trypsin at a final concentration of 0.01 [micro]g/[micro]L, and filtered through a C18 cartridge before being subjected to liquid chromatography (LC) with a 5%-50% acetonitrile gradient in 0.1% formic acid, followed by tandem MS (LC-MS/ MS; LTQ ThermoElectron
, San Jose, CA, USA).
Selenium in whole blood was analyzed at a commercial laboratory (Analytica AB, Lule[Angstrom], Sweden) with an inductively coupled plasma sector field mass spectrometer (ELEMENT, ThermoElectron
; Finnigan MAT, Bremen, Germany) monitoring m/z 78 in high-resolution mode (delta m/m = 11,000).
Flow of electrons is produced due to the effect of thermoelectron
emission of the heated cathode.
(Bear was later acquired by ThermoElectron
later formed a division for adult ventilator sales called Viasys.
In addition, this deal signals what may be a new period of divestments for ThermoElectron