The smoking habit has been found to be significantly correlated with the levels of lipid peroxidation as determined by the
thiobarbituric acid reactive substances (TBARS) concentration [16].
Evaluation of lipid oxidation: the
thiobarbituric acid reactive substances-test
Heparin,
thiobarbituric acid (TBA), trichloro acetic acid, 2, 4-dinitro phenyl hydrazine, 1-Choloro -2, 4-dinitrobenzene, nitroblue-tetrazolium, phenazinemethosulphate were acquired from Hi --media Laboratories, Mumbai, India.
Thiobarbituric acid reactive substances (TBARS) reflect lipid peroxidation due to increased oxidative stress and tissue injury.
Determination of
thiobarbituric acid reactive substances
The amount of lipid peroxidation is measured according to the concentration of
thiobarbituric acid reactive species.
Then, 1 mL of plasma was added to a 2 mL mixture of equal parts of 15% trichloroacetic acid (TCA), 0.25 M hydrochloric acid (HCl) and 0.4%
thiobarbituric acid (TBA) plus 2.5 mM butylated hydroxytoluene (BHT) and 0.1 mL of 8.1% sodium dodecyl sulfate (SDS), being heated for 30 minutes at 95[degrees]C in an oven.
Determination of MDA, measured as
Thiobarbituric acid reactive substances (TBARS) in liver homogenate.
This study was conducted to investigate the effects of starvation for 8 days on the serum metabolites and antioxidant enzymes,
thiobarbituric acid reacting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss).
In
thiobarbituric acid based tricyclic-heterocycles (1d-7d) 1H NMR spectrum of four NH signal split into two separate singlets in the range I' 11.81-11.64 and 11.69-11.48 ppm, while methine hydrogen was observed in the range of I' 6.03-5.80 ppm as a singlet.
Measurement of
thiobarbituric acid reactive substances (TBARS)
5-5 dithionitrobenzoic acid and
thiobarbituric acid and Trichloroacetic acid were purchased from Merck Chemical Co.
