Samples were sent for cultivation on blood, chocolate, Mc-Conkey agar and
thioglycolate broth.
All blood specimens were put into
thioglycolate broth and subcultured after overnight incubation on 5% blood agar, Mac Conkey agar and Chocolate agar plates and incubated overnight at 37[degrees]C aerobically.
The medium used for isolation and cultivation of strains grown on
Thioglycolate Agar (TGA) medium and was prepared as follows; 15 g/L pancreatic digest of casein, 5.5 g/L dextrose, 5 g/L yeast extract, 2.5 g/L sodium chloride, 0.5 g/L sodium
thioglycolate, 0.5 g/L L-cystine, 1 mg/L resazurin, 75 g/L agar with final pH 7 (29).
The samples were cultured in
thioglycolate medium, Blood agar and MacConkey's agar to detect aerobic and other bacteria.
difficile culture, as follows (4): 2 g of each sample were incubated in
thioglycolate broth for seven days under anaerobic atmosphere.
It is safe, is free of
thioglycolate acid (the corrosive lash perm chemical that dehydrates lashes and makes them frizzy), and will make your beauty routine simple and easy.
perfringens grown in fluid
thioglycolate medium (FTH), which contains sodium
thioglycolate and L-cysteine [21].
A
thioglycolate broth tube was used for enrichment of anaerobes.
Secondly, 0.9 gram of dibutyltin bis-(isooctyl
thioglycolate) (1.5 wt% in comparison with PVC weight) was then added to the above mixture.
Fungal cultures grown in
thioglycolate broth were used as primary inoculums.
hydrochloric acid, waiting for
thioglycolate, precipitated at 4[degrees]C for 4 h and centrifuged at 10,000g for 10 min.