Total
thiol content was determined as described by Aksenov and Markesbery (21).
A statistically very significant negative correlation was found between retinopathy with serum total
thiol levels (p<0.01).
Native
thiol and total
thiol values were measured using a new method developed by Erel and Neselioglu (17), and disulfide, disulfide/native
thiol, disulfide/total
thiol, and native thiol/total
thiol values were calculated.
With this new method, TDB tests including native
thiol (SH), disulfide (SS) and total
thiol (SH + SS) levels can be quantified simultaneously.
Reducible disulfide bonds were reduced to create free functional
thiol groups.
Troponin levels, native
thiol, total
thiol, and disulphide were compared among the groups.
Thiol is a sulfidryl (-SH) group-containing organic compound that can bind C atoms.
The homogenate was centrifuged at 18000 g for 20 minutes at 4 [degrees]C, and the resultant supernatant was used for the determination of oxidative stress markers (MDA and total
thiol groups).
The AA group had a significantly decreased native
thiol level (p<0.05) (Table 2).
Follicular native
thiol levels were decreased in the PCOS group (297.1[+ or -]37.3 [micro]mol/L) compared with the non-PCOS group (334.2[+ or -]72.5 [micro]mol/L; p=0.041), as well as the native thiol/total
thiol ratio (91.8%[+ or -]1.9% in the PCOS group, 95%[+ or -]2.3% in the non-PCOS group; p<0.0001).
Serum SH, total
thiol (TT), and SS levels were analyzed in these samples, and SS/SH, SS/TT, and SH/TT ratios were calculated according to the method of Erel and Neselioglu [11].
(3) The veterinary literature suggests that at least canines may be susceptible to a skunk
thiol induced hemolytic anemia, but no similar reports are noted in the human medical literature.