Immediately, after the solution was cooked, 6-10 drops of 1% thymolphthalein
indicator were added and the solution was immediately titrated with 0.1 N sodium hydroxide to the thymolphthalein
The alkaline phosphatase activity was measured based on the release of thymolphthalein
from the hydrolysis of thymolphthalein
The following Hammett indicators were utilized: phenolphthalein ([H.sub.-] = 8.2), thymolphthalein
([H.sub.-] = 10.0), 2,4-dinitroaniline ([H.sub.-] = 15.0), and 4-nitroaniline ([H.sub.-] = 18.4).
Rapid method for determination of alkaline phosphatase activity in human serum with thymolphthalein
The solution was then cooled to room temperature and titrated using thymolphthalein
as the indicator until a persistent color change was obtained.
The alkaline phosphatase present in the sample hydrolyzes the substrate thymolphthalein
monophosphate, releasing thymolphthalein
and inorganic phosphate.
Maternal blood placental alkaline phosphatase (PAP) activity was assayed by the Thymolphthalein
Monophosphate method (4) using the maternal sera samples heated at 65[degrees]C for 7 min.
This tendency was similar at the jejunum: 632; 1151; 749 and 405 ([micro]moles thymolphthalein
/min/mg protein) and ileum: 107; 225; 157 y 71 ([micro]moles thymolphthalein/min/mg protein) with the same concentrations of phytic phosphorus.
One strategy involves using an acid-base indicator such as thymolphthalein
, which has a deep blue color in an alkaline solution but is colorless in acidic conditions.
Analysis of breath tests: Expired [sup.14]C[O.sub.2] was collected through a calcium chloride column into a trapping agent consisting of 4 ml methylbenzethonium hydroxide and a pH colour indicator (thymolphthalein