3x3 cm plates of both TET2-pCDNA3 and pCDNA3 stable transfect
cells were grown with GF H4034, with control plates of the stably transfected
lines grown without GF H4034.
Electroporation may also penetrate to transfect
cultured organs or whole tissues of live organisms.
Recombinant VEGF-shRNA plasmids were transiently transfected
into HTF cells, and shRNAs were screened using a pSLIK miRNA-based vector that lacked VEGF interference sequences as a negative control.
Whole-cell patch-clamp recordings of miniature excitatory postsynaptic currents (mEPSC) were obtained from transfected
cultured hippocampal neurons treated with A[beta] oligomers on DIV 12-13 [41, 43].
Benitec recently announced that it has agreed to fund a second clinical trial at the City of Hope using the same triple vector to transfect
T cells in HIV/AIDS patients who have failed HAART therapy.
This is accompanied by significant increase of Ngn3, insulin, Glut2, Kir6.2, SUR1, and VDCC, as compared to cells transfected
with control siRNA (Figure 3(b)).
Jurkat-TAg cells were transfected
with an NFAT-luciferase plasmid plus a pgalactosidase ([micro]-gal) reporter plasmid as described [20, 21].
The rate of gap-filling in cells transfected
with PCNA and XRCC1 was slower than with PCNA alone (Figure 8(c), compare the curve of closed circles and the curve of open circles).
Cytosolic-free [Ca.sup.2+] was monitored in digitonin-permeabilized H9c2 transfected
H9c2 cells were transfected
with PPRE3-luciferase reporter and Ptk-RL and PPAR[gamma] plasmids as indicated.
MC3T3-E1 cells were transfected
with miR-155 overexpression plasmids via the lentiviral transfection method.