transfection


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transfection

[trans′fek·shən]
(genetics)
Infection of a cell with viral deoxyribonucleic acid or ribonucleic acid.
References in periodicals archive ?
qRT-PCR was performed to detect the expression levels of HULC, miR-130b, and FOXM1 in GH3 cells after relevant transfection. Briefly, total RNAs in GH3 cells were isolated using TRIzol[TM] Plus RNA Purification kit (Invitrogen).
Having transfection grade plasmid DNA available is critical to today's high value biopharmaceutical applications and working with Macherey-Nagel's proven anion-exchange technology will allow to develop a cost effective automation solution for this important process, added Piero Zucchelli, CEO and co-founder of Andrew Alliance.
Efficient transfection of the CRISPR construct into certain cells (e.g.
ST cells were plated on 24-well plates for 24 h before transfection. miR-375 mimics plasmids (1 [micro]g) containing the RLFWT (1 [micro]g), HIGD1A-WT (1 [micro]g), RLF-MUT (1 [micro]g), HIGD1AMUT (1 [micro]g) genes or luciferase vector (pmiR-RB-Report) (1 [micro]g) were co-transfected with 2 [micro]L of FuGENE HD (Roche, Switzerland) in triplicate for 36 h.
Transfection of plasmid DNAs into HEK-293T cells using Lipofectamine 2000
Upon completion of the incubation time, the lipoplex solutions were used in the transfection of the HEK 293-T cell line and for the in vivo experiment.
Low molecular weight PEI has low cytotoxicity but also has a low transfection efficiency [7-9].
Nevertheless, we were able to establish a transfection protocol with a plasmid encoding only SOX2 that resulted in increased RMCMO proliferation and redifferentiation potential.
Our group showed that functional depression of monocyte from trauma patients went along with reduced NLRP1 mRNA expression and moreover that restoring NLRP1 via transfection recovered the functional activity of monocytes [19].
To manipulate the expression levels of miR-34a, cells were transfected with miR-34a mimic or miR-34a inhibitor, along with their corresponding negative controls, using HiPerFect Transfection Reagent (Qiagen, Shanghai, China).
These innovations also served as the basis for the company's transfection formulations and nucleic acid labeling and conjugation chemistries.
Transfection is a process of inserting nucleic acid into somatic cells or cell lines using various chemical or physical methods.