transfection

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transfection

[trans′fek·shən]
(genetics)
Infection of a cell with viral deoxyribonucleic acid or ribonucleic acid.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
A transfection plasmid was inserted using FuGENE HD (Roche, Switzerland) according to the manufacturer's instructions.
HCT-116/OR cells with different transfections were incubated with different doses of oxaliplatin for 24 h, followed by cell viability assay (B) and apoptosis evaluation (C).
These innovations also served as the basis for the company's transfection formulations and nucleic acid labeling and conjugation chemistries.
Caption: Figure 2: PPRE-H2B-eGFP and PPRE-pNL1.3[secNluc] transfections of VCT: new models to visualize and quantify the activity of PPAR[gamma].
The cationic polymer polyethylenimine (PEI) has been widely used for nonviral transfection and has an advantage over other polycations in that it combines strong DNA compaction with an intrinsic endosomolytic activity [11].
Thereby, the optimization of the TGE technique for Chinese hamster ovary (CHO) as the dominant host for the production of biotherapeutics is of great interest to reach the values for Human Embryo Kidney-293 (HER-293) cells in terms of transfection efficiencies and production titers.
Statistical analysis of the data obtained in the evaluation of transfections was determined between groups using ANOVA test and by the Tukey nonparametric test.
Forty-eight hours following transfection, the medium was aspirated, and the cells were washed 2X with 4 mL ice cold PBS.
There have been no previous reports of somatic gene transfer to the fish brain; therefore, it was necessary to optimize injection and transfection conditions for the adult goldfish (Carassius auratus).
Transient transfection studies with a [beta]-globin promoter and an heterologous reporter gene have shown that promoter mutations can decrease transcription (2) and are then associated with the [beta]-thalassemia phenotype, as illustrated by the -30T [right arrow] A mutation (3).
Note: TransIT[R]-Lenti Transfection Reagent is only recommended for adherent cell transfections (e.g., HEK 293T/17) and is not recommended for lentivirus generation in suspension cell types.