Sixty individuals were separately euthanized with
tricaine methane sulfonate (MS-222) and stored at -20AdegC for the entire carcass body before the start of the experiment.
Salamanders were caught by hand and anaesthetised using
tricaine methanesulphonate (MS-222), one of the anaesthetics most commonly used in the field sampling of amphibians (e.g.
The drugs used for immersion anesthesia for elasmobranchs are the following: benzocaine, etomidate, metomidate, halothane-oxygen-nitrous oxide, oxygen, quinaldine and
tricaine methane sulfonate (MS-222) (Stamper, 2004; Stamper, 2007).
Sampled fish were sacrificed with an overdose of
tricaine methanesulphonate (MS-222; 1 g [L.sup.-1]).
Euthanasia was performed in accordance with the American Veterinary Medical Association Guidelines for the Euthanasia of Animals (AVMA, 2013); primary euthanasia was exposure to buffered, pharmaceutical-grade
tricaine methanesulfonate (MS-222), followed by decapitation as a secondary method.
Several substances and combinations of substances, such as alcohol, ether, barbiturates, quinaldine,
tricaine methanesulfonate (MS 222), chlorbutanol and benzocaine have been used to induce anesthesia in fish.
At 48 hpf, embryos were screened for malformations, and the morphologically normal animals were euthanized by overdose of buffered
tricaine methanesulfonate (MS-222, 200-300 mg/L) by prolonged immersion and monitored under a dissecting microscope until their hearts stopped beating (approximately 15 min).
On November 17, 2015, after several days of recovery from the artificial spawning procedure, 55 fish were anaesthetized with
tricaine methanosufonate (MS-222) and surgically implanted with MCFT2-3L (5) radio transmitters (Lotek Wireless, Inc., Newmarket, Canada).
At the appropriate time-point following environmental or pharmacological stimulus, each fish was anesthetized in
tricaine methanesulfonate (MS 222; 300 [micro]g/mL) for 4 min and then euthanized by decapitation.
Saczk, "Determination of benzocaine and
tricaine in fish fillets using BIA with amperometric detection," Electroanalysis, vol.
Animals were anesthetized via immersion in 1%
tricaine mesylate (MS222) buffered to a pH of 7.
At each sampling event, fish were killed with an overdose of
tricaine methanesulphonate (MS-222).