tris buffer


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tris buffer

[′tris ‚bəf·ər]
(organic chemistry)
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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The cells were harvested by centrifugation at 8000 xg for 5 min, washed with a Tris buffer (20 mM Tris-HCl (pH8.5), 1mM EDTA, 1mM 2-mercaptoethanol, and 1mM PMSF).
Then the sections were washed with distilled water and dipped in Tris buffer solution.
We conducted reactions in the presence of three different buffers: (1) Tris buffer with no additions; (2) Tris buffer amended with 5 mM Ca[Cl.sub.2]; (3) Tris buffer amended with the [Ca.sup.2+] chelator 5 mM EGTA (ethylene-glycol-bis ([beta]-aminoethyl ether)-N,N,N',N' tetraacetic acid).
Twenty (20) mg of 10,12-pentacosadiynoic acid was dispersed in 4 ml of a Tris buffer (pH ~ 11).
A solution consisting of 0.010 mol/L 2-amino-2-hydroxymethyl-propane-1,3 diol (TRIS) pH 8.0 buffer (TRIS buffer) was used to dissolve the components and was used as the blank.
7 ml of extra cellular phytase obtained from ultra filtration contain 200 mg/ml protein was applied to a sephadex G-100 column chromatography (108 x 2.6 cm) which had been equilibrated with 0.1 M Tris buffer at pH 7.0 at 11[degrees]C.
In this study, influencing factors on the precipitation rate depending on the constituents of bio-based material comprising yeast, glucose and calcium acetate mixed in tris buffer solution was examined for improving the rate of initial reactions.
B, Optimal epitope retrieval using 30 minutes of Tris buffer, pH 10.
Cada pajuela fue descongelada en un bano de agua a 38[degrees]C por 60 s y el contenido de cada una vaciado cuidadosamente en diferentes tubos con medio de descongelacion [Tris Buffer a un volumen de 1:2 (v/v)] y centrifugando a 300 g por 5 min, el sobrenadante fue eliminado y el pellet resuspendido en medio Tris Buffer a una concentracion de 2x106 espermatozoides/mL.
To determine whether meprins are capable of proteolytically processing OS-9 present in kidney tissue, activated purified forms of recombinant homomeric meprin A ([alpha]-[alpha]) and meprin B ([beta]-[beta]) were incubated with 60 [micro]g of cytosolic-enriched kidney proteins from meprin [alpha][beta] double KO mice (which lack endogenous meprins) in Tris buffer (20 mM Tris and 150 mM NaCl, pH 7.5) at 37[degrees]C for 4h.
Solutions were prepared with Tris Buffer in the exact same manner and repeated for all glass batches.