Specimens have been tested at 1 day and 7 days from the end of trituration
and should be stored at temperature of 37 [+ or -] 1[degrees]C until the test was done.
At the time of surgery, the transduced spheres were dissociated by enzymatic digestion and mechanical trituration
. Then, 1 x [10.sup.4] cells were re-suspended in 10 [micro]L aliquots of culture medium for transplantation.
Some leaves were submitted to a mate-type yerba-mate processing, which involved the following steps: (i) roasting at 180[degrees]C for 5 minutes at 20 rpm in a prototype laboratory equipment, previously described (Valduga, Finzer, & Mosele, 2003); (ii) drying in an air-circulation oven at 70[degrees]C, for about 12 hours (moisture < 3%); (iii) trituration
in a knife mill, followed by homogenization in a Tyler-series sieve with mesh 16 (< 2.36 mm).
At present, two major approaches have been applied to isolate skeletal muscle satellite cells, the first approach is to break down the connective tissue network and myofibers to release the muscle satellite cells based on the mincing, enzymatic digestion and repetitive trituration
of the muscle mass.
and anti-solvent crystallization methods have been developed to efficiently afford the desired ligands in good telescoped yield over four total steps without additional purification.
Untreated sewage inputs to the lake and water hyacinth trituration
in parts of Lago de Patzcuaro have caused tremendous input of oxygen-demanding wastes that degrade water quality, changing productivity (Xie et al., 2004).
The resulting marrow pellet was dispersed by trituration
, and the cell suspensions from all bones were combined in a centrifuge tube.
In addition, the thick muscle fibers which arranged in two layers demonstrated the powerful trituration
Scholander pressure bomb was found to be useful in extraction of diverse bacterial genera from xylem tissues in contrast to trituration
methods that yielded higher number of Gram-positive rod shaped bacteria .
The calculated amount of carrier and coating material was added to the dispersion and blended in a porcelain mortar avoiding excessive trituration
and particle size reduction as described by Spireas et al., .
in adult carabid beetles (Coleoptera: Carabidae).
Amalgam does not show any significant dimensional change except when it is contaminated during trituration