trypan blue


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trypan blue

[′tri‚pan ′blü]
(materials)
An acid diazo dye of the benzopurpurine series used as a vital stain.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
Cellular viability of DPSC and SHED was observed using trypan blue dye exclusion test indicating the cell membrane integrity was preserved during cryopreservation as shown in Fig.4.
After sh-HULC or pc-HULC transfection, A, the expression of HULC in GH3 cells, B-E, the viability, migration, and invasion of GH3 cells, F, the expression levels of E-cadherin, N-cadherin, vimentin, and snail in GH3 cells, G, the apoptosis of GH3 cells, H, the expression levels of PARP, cleaved- PARP, pro-caspase 3, cleaved-caspase 3, pro-caspase 9, and cleaved-caspase 9 in GH3 cells, and I and J, the concentration of prolactin (PRL) and growth hormone (GH) in culture supernatant of GH3 cells were assessed using qRT-PCR, trypan blue staining assay, MTTassay, two-chamber transwell assay, Guava Nexin assay, western blotting, and ELISA.
After 48 hr, viability of oocytes was estimated after vital staining with trypan blue (Sigma-Aldrich, USA).
Each type of cells was treated with L at concentrations ranging from 10 [micro]M to 500 [micro]M for 24 hours, and the cell viability was quantified using a trypan blue exclusion assay.
Except the EB dye, several other dyes are useful for identification of sentinel lymph nodes and include Chicago sky blue, Patent blue, and Trypan blue [27, 77].
Finally, viable parasites after exposure to caspofungin were determined by the trypan blue assay.
At 24 h posttransfection, cells in cocultures, treated or not treated with trypan blue 0.04% in PBS in order to quench extracellular fluorescence, were detached with trypsin/ethylenediaminetetraacetic acid (EDTA) and finally resuspended in 50 [micro]l PBS.
Commonly used dyes include indocyanine green (ICG), trypan blue (TB), brilliant blue G (BBG), and acid violet 17 [58].
Cell viability was assessed by trypan blue and cytotoxicity experiments were done using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay.