merolae lacks a typical cytoskeleton, the tubulin
function in this organism is mostly limited to the formation of mitotic spindles.
TUBB3 and K1F21A primer sequences for conventional PCR Gene (exon/s) Forward sequence Reverse sequence tubulin
beta-i(l) CAGCTCCTCTGGGAGACA CATCCCTTTGTTGCAGGTT tubulin
beta-3(2) GAGGGCTAAAAGGCTTCACA GGTGCTGAGACCTGGTCAGT tubulin
beta-3(3) CGGGCACAGAATTCAGAAA TCATGTGAGGAGCTGACCAT tubulin
beta-3(4) TGCCCTTGGGATGTTCAG GGGATCCACTCCACGAAGTA tubulin
bcta-3(4) GTTCGATGCCAAGAACATGA AGCTCTTCTTGCCTGTCCAC K1F21A(8) TTTTAGCATTTTAGGTGCTTTT AAAGTGCCAGCCTTAGATGT KIF21A(20-21) TGTTGTACTTAAATGAAAAAATGGCTC AGAGAAATCTGAAAAGCAAGCAGG Gene (exon/s) Amplicon size tubulin
beta-i(l) 485 bp tubulin
beta-3(2) 272 bp tubulin
beta-3(3) 300 bp tubulin
beta-3(4) 846 bp tubulin
bcta-3(4) 862 bp K1F21A(8) 306 bp KIF21A(20-21) 794 bp
Microtubules are the key components of the cytoskeleton of eukaryotic cells and have an important role in various cellular functions such as intracellular migration and transport, cell shape maintenance, polarity, cell signaling, and mitosis.5 The real biologic basis by which tubulin
inhibition would result in termination of a sustained monomorphic arrhythmia; cannot be fully explained.
Duocarmycins are able to exert their mode of action at any phase in the cellular cycle, whereas tubulin
binders will only attack tumor cells when they are in a mitotic phase.
This distinction was not possible because of the limitations of gamma tubulin
Structure of the alfa-beta tubulin
dimer by electron crystallography.
Immunohistochemical staining of Tubulin
after fixation with NBF, Alcoholic Formaline, AFA, Bouin's, Carnoy's and Clarke's solutions.
Single cell suspensions were then stained with appropriate antibodies as follows: mouse antinestin (1: 50, Abcam), rat anti-CXCR4 (1: 50, BioLegend), and rabbit anti-[beta]III tubulin
(1: 10, Epitomics).
A complete transcriptome analysis on biopsies of DMD patients has also revealed an upregulation of several X-ROS-related transcripts, including NOX2 and nine different tubulin
The article was found to contain images with the following concerns: There are irregularities in the background in Figures 1(a) and 1(b); the tubulin
controls in Figures 1(a) and 1(b) are the same; Figure 3(b), Cyclin B (72 hrs) is the same as Figure 6(b), TGF betal (24 hrs); Figure 5(c), the lanes in the TGF-alpha rows at 6 hours and 72 hours do not align.
Our group has reported, for the first time, the presence of HDAC6 in sperm and demonstrated its tubulin
specific deacetylase activity and its role in sperm motility in Holtzman rat .
Their topics include post-translational modifications of tubulin
and cilia, the central apparatus of cilia and eukaryotic flagella, transition zone migration: a mechanism for cytoplasmic ciliogenesis and postaxonemal centriole elongation, primary cilia and mammalian hedgehog signaling, G-protein-coupled receptor signaling in cilia, ciliary mechanisms of cyst formation in polycystic kidney disease, sperm sensory signaling, cilia and ciliopathies in congenital heart disease, photoreceptor cilia and retinal ciliopathies, and the evolution of cilia.