uranyl acetate


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uranyl acetate

[′yu̇r·ə‚nil ′as·ə‚tāt]
(inorganic chemistry)
UO2(C2H3O2)2·2H2O Poisonous, radioactive yellow crystals, decomposed by light; soluble in cold water, decomposes in hot water; loses water of crystallization at 110°C, decomposes at 275°C; used in medicine and as an analytical reagent and bacterial oxidant. Also known as uranium acetate.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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Another two washes with buffer were performed before uranyl acetate staining (1 % uranyl acetate in 10 % ethanol for 8 hours).
Ultrathin sections were taken with LKB ultramicrotome III and stained with uranyl acetate and lead citrate.
Samples were stained en bloc within a range of 1/5 stock RR solution and 2% uranyl acetate (22), then dehydrated in a ascending alcohol series and embedded in epon 812.
Reagents and poison: Electron microscopy reagents (EPON EMBed-812 Kit, uranyl acetate, osmium tetroxide, lead citrate and glutaraldehyde) were purchased from Electron Microscopy Sciences (Hatfield, PA, USA).
Post-stain procedures for 100-nm sections observed with electron microscopy varied, with the sample being treated either with (1) 2% aqueous uranyl acetate (15 min) and 0.5% aqueous lead citrate (1 min) with 3 washes of 20 s with DI water in between; (2) only 2% aqueous uranyl acetate (15 min); or (3) only 0.5% aqueous lead citrate (1 min).
(e) Ultrastructural examination shows osmophilic globular hemoglobin casts in tubular lumen (uranyl acetate and lead citrate fixation, 10,000x).
For visualization using TEM, uranyl acetate was used as a negative staining reagent for the liposomes [6].
Poly(vinyl alcohol) (PVA, Mw of 15kDa), uranyl acetate dehydrate (98%), dimethyl sulfoxide (DMSO, 99.5%), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), fluorescein isothiocyanate (FITC), and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoli-um bromide (MTT) were supplied by Sigma-Aldrich (St.
(a) H&E stain, x40; (b) GiemsaOrcein stain, x100; (c) and (d) uranyl acetate & lead citrate, original magnification x 13.000.
After uranyl acetate staining, the pellet was rinsed with 1.5 ml of distilled H2O immediately and was then repelleted by centrifugation.
12 [micro]l of the dilutions were allowed to adsorb on carbon coated copper grid for 1 min followed by negative staining with aqueous uranyl acetate (0.5%).