The results showed that the disappearance of Nissl-stained profiles from the ventral horn
reflects death of these cells and is not caused by alterations in the staining properties of injured motor neurons.
Astrocytes in the Spinal Ventral Horn Are Activated by Peripheral Nerve Injury in Early Stages and Reverted in Later Stages.
Then, FG retrograde labeling was applied to show the motoneurons in the spinal ventral horn. Incorporating the GFAP immunostaining, a widely accepted specific marker of activated astrocyte , we found the activated astrocytes surrounded the FG-labeled motoneurons (Figure 1(a)).
In WT rats, RAGE shows an evident neuronal localization and marks motor neurons in the ventral horn
region, as indicated by coexpression with the typical motoneuronal marker ChAT (Figure 2, WT--GM insets), with no overlapping signal with GFAP (Figure 2, WT--GM).
This speculation is reinforced by the present results of MAP2 immunostaining, which demonstrated RGE-mediated suppression of neuronal degeneration in and around the central canal and ventral horn
in cases of SCI.
The GABA-ir neurons, which were stained intensely, were numerous throughout the cortex region of the ganglion, especially in the upper and lower third of the dorsal and ventral horns
of the ganglion (Fig.
Our analysis of the inflammatory responses in the spinal cord of spinal rats with and without a graft revealed that in the ventral horn
of the lumbar segments (6 mm below the graft site--the shortest distance below the graft that MNs of TA and GM muscles were collected), there was no increased level of astrocytes and no presence of activated microglia.
Caption: Figure 3: Tail nerve electrical stimulation and electroacupuncture treatment can protect the motor neurons in the ventral horn
of the lumbar spinal cord.
The point intercepts stereological tool was employed to obtain the areal fraction  in the sampled region of the ventral horn
of the spinal cord rats, bilaterally, as described elsewhere [20, 43, 44].
Nonsuppurative myelitis is oriented on the ventral horn
involving neuronal degeneration with satellitosis (black arrow), neuronophagia (arrowhead), and glial nodule formation (blue arrow), accompanied by perivascular mononuclear cell I accumulation and gliosis.
Malformations of the central nervous system included bilateral hydrocephalus, cerebellar hypoplasia, and micromyelia, characterized by the presence of few neurons in the ventral horns
and moderate to severe bilateral reduction of white matter in the ventral and lateral funiculi.
Abnormalities were confined to the gray matter and involved both the dorsal and ventral horns