xanthene dye


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xanthene dye

[′zan‚thēn ′dī]
(organic chemistry)
Any of a family of dyes related to the xanthenes; the chromophore groups are (C6H4).
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The xanthene dyes Rose Bengal, erythrosin B, eosin Y, and fluorescein, the reagents 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 1-octanol, were purchased from Sigma-Aldrich and were used without further purification.
In order to study the intrinsic cellular toxicity of xanthene dyes, the cells were harvested using trypsin and incubated overnight for complete cell adhesion in 96-well plates at a concentration of 1 x 105 cells [mL.sup.-1] (2mL [well.sup.-1]).
The chemical structure of the four xanthene dyes Rose Bengal, erythrosin B, eosin Y, and fluorescein studied in this work (Figure 1) was also investigated with Density Functional Theory (DFT) as implemented in Gaussian 03 package [47].
It is important to comment that the incubation time affected the xanthene dyes cytotoxicity, where the decrease of the incubation time leads to an increase of the [IC.sub.50] value, suggesting that more dyes accumulate in the cell.
Arakawa, "Photosensitization of porous Ti[O.sub.2] semiconductor electrode with xanthene dyes," Chemistry Letters, vol.
Phloxine B and Eosin Yellow are halogenated photoactive xanthene dyes, that are FDA approved for use in human cosmetics and drugs.
Several simple organic dyes though, and especially xanthene dyes (Eosin Y, Rose Bengal, etc.), yield efficiencies comparable to those achieved with ruthenium complexes, especially when used to sensitize ZnO films [7-9].
In general, xanthene dyes such as Rose Bengal, Eosin Y, Mercurochrome, and Eosin B have a better performance when used to sensitize ZnO rather than Ti[O.sub.2] cells [7].