According to the results, among the ZnO treated plants, 1 uM ZnO caused the lowest ROS level (measured by
Xylenol orange, Fig.
Lipid hydroperoxide measurement by oxidation of Fe+ 2 in the presence of
xylenol orange.
Methods: A tissue equivalent material, ferrous benzoic
xylenol orange gel, was prepared using gelatine, ferrous ammonium sulfate, sulfuric acid,
xylenol orange tetrasodium salt and benzoic acid.
Bovine serum albumin, acrylamide, N,N'-methylene-bisacrylamide, trichloroacetic acid (TCA), FeS[O.sub.4], sulfuric acid, cumene hydroperoxide (CHP), butylhydroxy-toluene, methanol,
xylenol orange, di-nitrophenylhydrazine (DNPH), guanidine, ethanol, ethyl acetate, hydrochloric acid, Coomassie[R] Brilliant Blue R-250, thioglycolic acid, NBD-Cl, and o-phthalaldehyde (OPA) were purchased from Sigma-Aldrich (St.
All miniature pigs were injected intravenously (10ml/min) with three different fluorochromes
xylenol orange (6%, 2-5g/animal), calcein green (1%, 0.8-1.5 g/ animal), and alizarine complexone (3%, 1-1.5 g/animal) 14, 28, and 84 days after implantation.
This assay is based on the ferrous oxidation of
xylenol orange following the addition of methanol and butylated hydroxytoluene (BHT).
[30] explored the product analysis of impregnated lignite coal with sulphided molybdenum catalyst and exhibited increment in yield of tar up to about 63.9% as well as light aromatics such as BTX, phenol, cresol, and
xylenol (PCX), and naphthalene also increased during the catalytic multistage hydropyrolysis (MHyPy) as compared to the traditional hydropyrolysis.
This process was monitored with
xylenol orange (i.e., it changes from yellow to blue when it reacts with [Fe.sup.+3]) and the absorbance was evaluated with a spectrophotometer (S-20 Vis, Boeco Germany) set at 560 nm.
After, 30 [micro]L of the supernatant was added to 270 [micro]L of reaction medium [4mM butylated hydroxytoluene (BHT), 250 mM FeS[O.sub.4], 25mM [H.sub.2]S[O.sub.4], and 100mM
xylenol orange], solubilized in methanol, and incubated for 30 minutes at 25[degrees]C.
1-Pyrenebutyric acid, diethylenetriaminepentaacetic acid (DTPA), 1,3-diaminopropane, N-hydroxysuccinimide, 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDC), gado-linium (III) chloride, and
xylenol orange were purchased from Sigma-Aldrich (St.
Ferric ions form a colored compound with
xylenol orange in the acidic media.
The method is based on the principal of the oxidation of ferrous ion to ferric ion in the presence of various oxidants in the sample and the measurement of the ferric ion by
xylenol orange.
