In our experiment, ferrous benzoic
xylenol orange (FBX) gel was prepared using gelatine (from bovine skin, Type B), ferrous ammonium sulfate (Aldrich ammonium iron (II) sulfate hexahydrate, 99% American Chemical Society (ACS) reagent), sulfuric acid (Sigma-Aldrich),
xylenol orange tetrasodium salt (Sigma-Aldrich) and benzoic acid (Sigma-Aldrich) formulated in 1998 by Kelly RG16 and others.
The second, the content of La has been measured with using
xylenol orange as indicator by EDTA titration.
The ferric ion produces a colored complex with
xylenol orange in an acidic medium.
MEASUREMENT OF OXIDATIVE STRESS PARAMETERS: The oxidative stress was evaluated by estimating the amount of oxidant load of lipid peroxides was determined by ferrous oxidation products in
xylenol orange assay in conjunction with triphenylphosphine version 2 (FOX2 assay) (18).
Ferrous ion oxidation in the presence of
xylenol orange for detection of lipid hydroperoxide in low density lipoprotein.
1994; ferrousoxidation of
xylenol orange method I: FOX I).
Ferrik iyonlar asidik ortamda
xylenol orange ile renkii bir kompleks olustururlar.
6 mg
xylenol orange was added while stirring to make the final working reagent (250 [micro]M ammonium ferrous sulphate, 100 piM
xylenol orange, 25mM [H.
Hydroperoxide levels: An aliquot of cytosol or mitochondria (100 [micro]g protein) was added to 1 ml FOX reagent (100 [micro]M
xylenol orange; 250 [micro]M ammonium ferrous sulphate; 100 [micro]M sorbitol; 25 mM [H.
Of the substituted phenols, the most important are alkylated phenols like m-cresol which reacts with formaldehyde more readily than phenol, and 3,5
xylenol which reacts even faster than does m-cresol.
A working color reagent was prepared by mixing 100 ml distilled water containing 100 mM sorbitol and 125 [micro]M
xylenol orange (Sigma, USA) with 1 ml of 25 mM ferrous ammonium sulphate prepared in 2.
The labile zinc in this case was detected in the rinse water by colorimetry with the metallochromic dye
xylenol orange.
