Previous studies have suggested that embryos with 3PN, which develop from ICSI zygotes
, are endowed with mechanisms to correct triploidy to diploidy.
Following IVF, presumptive zygotes
were cultured in SOFaa embryo culture medium supplemented with or without antioxidants (a mixture of 1 mM L-glutathione reduced and 1,500 IU superoxide dismutase).
Figure 1J-N shows the zygote
at early anaphase of the first meiosis viewed from the animal pole.
Successful applications of ssODN have been demonstrated in various animal models through direct injection of CRISPR/Cas9 components into zygotes
[28-30] or in human ESCs and iPSC for modeling human diseases [31-33].
When researchers injected the Cas9RNA protein targeting the mutation into already fertilized zygotes
, they found that 67% of the resulting embryos had two normal copies of the MYBPC3 gene.
High levels of ROS represent a major contributing factor in vitro to developmental blockage in zygotes
They suggest possible alternative approaches to zygote
injection that would avoid both of those pitfalls.
In mouse embryos, the gene is active in zygotes
; its RNA then dips in abundance but rises again at the eight-cell stage.
A human embryo is the product of a fertilised egg, from the zygote
until the fetal stage  or 'the developing organism from fertilization to the end of the eighth week.
In each of these cases, the mitochondria in the egg or the donated zygote
replace defective mitochondria and protect the child from related diseases.
In the July 6 samples after double fertilization the zygote
remained undivided which was located at the micropylar pole (Table 1).