Our findings also show that regulated death at an acidic pH relies not only on the function of RIPK1, caspase-8, but also PARP-1, implicating parthanatos [41] as well as apoptosis and necroptosis.
The RIPK1 inhibitor Nec-1s, which blocks necroptosis, was added to cultures at pH 7.4 (Figures 2(a) and 2(d)), pH 6.7 or pH 6.0 (Figures 2(b), 2(c), and 2(d)).
Death in endothelial cells at an acidic pH relies on the function of RIPK1 and caspase-8 (Figures 2 and 3).
RIPK1, RIPK3, caspase-8, and PARP-1 all appear to be necessary to induce necrotic death with TRAIL at low pH.