Preparation of ODEX: taking proper amount of DEX precision, DEX was dissolved using phosphate
buffer solution (pH 4.5).
USP guidelines were used to determine the suitability of system which consists of optimized conditions, i.e., merging of ammonium acetate
buffer solution pH 7.6 and acetonitrile 50:50% v/v as mobile phase at a flow rate of 0.5 mL/min over Agela C18, 125 mm x 4.6 mm, 5 um.
TX working solutions for voltammetric inquiries were prepared by direct dilution of the stock solution with 1 M acetate
buffer solution at pH 5.5 containing a constant amount of methanol (20% V/V).
Each kit contained four 4 mL glass vials of quench
buffer solution, comprising 8 mol/L guanidine-HCl and 0.4 mol/L sodium phosphate in [H.sub.2]O (pH 3.1 [+ or -] 0.02).
The experiments were performed in a phosphate
buffer solution adjusted to a pH of 6.7.
Standard solutions 10 mmol [L.sup.-1] of AML and ATOR were prepared before the use in a BR
buffer solution (pH 4.0) containing 10% methanol (v/v).
In our experiments, the addition of hirudin to both the
buffer solution containing purified human thrombin and to the NHS sample completely suppressed the degradation of cTnT, which suggests it is thrombin that is responsible for the cTnT cleavage in serum (Fig.
McDougall
buffer solution: McDougall
buffer solution was prepared as per method reported by (McDougall.