In our review, in all cases of culture-negative synovial fluid, at least one of the following was present: risk contact, skin lesions, previous respiratory symptoms, positive nasopharynx and blood culture, or presence of Gram-negative
diplococci in a Gram stain (Table 2).
The second swab samples were utilized for smear preparation in Gram staining to see Gram-negative intra or extracellular
diplococci under bright field microscope.10
Isolation of bacteria from the respiratory system of both healthy and diseased camels has been reported by a number of researchers; Arora and Kalra [6] reported isolation of Klebsiella pneumoniae and
diplococci from camel pneumonic lungs in India.
pneumoniae is gram-positive
diplococci, having kidney shaped morphology which is examined by gram staining technique.
An autopsy was performed on January 30, and on January 31, the medical examiner reported Gram-negative
diplococci in the cerebrospinal fluid (CSF).
(7) For gonorrhea it was required isolation of Gram-negative intracellular
diplococci by culture from a clinical specimen (a urethral smear obtained from a male or an endocervical smear obtained from a female).
As a result of the studies conducted on organs taken from sick and fallen animals and also on fecal masses of healthy animals, we selected and identified the following cultures that are, under certain conditions, capable of causing intestinal diseases in the young stock of farm animals: 445, Salmonella; 155, Escherichia; 56, Klebsiella; 72, streptococci (
diplococci); 62, staphylococci; and 12, olm (Table 1).
Lactococcus and Enterococcus are cocci Gram-positive that often occur in pairs (
diplococci) or short chains and are difficult to differentiate from streptococci on physical characteristics alone.
The discharge results were negative for trichomonads and Gram-negative
diplococci were not visible within phagocytes, direct microscopic fungi tests were negative, and cultures for general bacteria, gonococci, Ureaplasma urealyticum , and fungi showed negative results.
gonorrhea was diagnosed by culture (modified Thayer-Martin medium) and/or positive Gram-stained of endocervical swabs for Gram-negative
diplococci. H.
Gram stain of the CSF revealed no inflammatory cells with numerous gram-positive
diplococci and rare to few round yeast cells.