In prokaryotes, modification of DNA prevents cleavage by the cognate
restriction endonucleases [2].
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12
restriction endonucleases cleaved the amplicons with more than one haplotype.
Digestion of amplified allele products with a
restriction endonuclease can also provide very discrete typing results.
Polymorphism in mitochondrial DNA of humans as revealed by
restriction endonuclease analysis.
Thirteen
restriction endonucleases generated a total of 28 fragments; four of the
restriction endonucleases (BamHI, EcoRI, PstI, SalI) made no cuts, while another
restriction endonuclease (BglII) resulted in a single cut (Table 1).
Chromosomal digest by low-frequency cutting
restriction endonuclease and pulse field gel electrophoresis revealed an identical pattern, suggesting a probable common origin.
Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and
restriction endonuclease analysis.
The
restriction endonuclease digest of the 511 bp COI PCR product resulted in 2 bands of 318 bp and 193 bp.